Proteomics Research Based on SPR Technology

The overview of proteomics research

The proteome refers to the collection of all corresponding proteins expressed by the genome, that is, the existence and activity of all proteins in cells, tissues or organisms. Proteomics essentially refers to the study of protein characteristics at a large-scale level, including protein expression levels, post-translational modifications, and protein-protein interactions. Through the related research of protein self-omics, it is possible to obtain an overall and comprehensive understanding of the process of disease occurrence and cell metabolism at the protein level. Proteomics is a science that studies life phenomena and laws as a whole and dynamically at the protein level. It is an important organic part of systems biology.

• The process of proteomics analysis

As shown in the figure below, the routine process of proteomics analysis includes protein extraction, purification, and structural research. Cells or tissues are processed through various physical and chemical techniques to extract total protein. According to the physical and chemical properties of peptides, different chromatographic techniques can be used to separate the protein of interest. X-ray crystallography and nuclear magnetic resonance have been widely used for structural analysis and functional characterization of proteins. In addition, the analysis of protein-protein interactions and interactions between proteins and other biologically active molecules occupies an important position in proteomics analysis. On the one hand, molecular interactions can be used to identify and quantify the total protein content present in cells, tissues or organisms. On the other hand, molecular interactions help analyze the effects of interactions between proteins and other substances. Proteomics supplements other omics technologies such as genomics and transcriptomics. It clarifies the identity of biological proteins while helping people understand the structure and function of specific proteins.

Fig.1 Schematic diagram of proteomics analysis (Aslam, B, et al., 2016)

Surface plasmon resonance (SPR) in proteomics analysis

Proteomics analysis is a complex and dynamic process. As shown in the figure below, our SPR technology platform is mainly able to realize high-throughput interaction analysis between proteins and various molecules. In addition, through molecular interaction analysis, SPR technology can quickly identify the types of proteins in the proteome and accurately quantify their content. Our SPR technology platform can help you to further reflect the relevant information of genomics through the dynamic real-time analysis results of proteomics, and help you to further explore the biological mechanism. As a huge screening library, the proteome has been widely used in the first stage of new drug development. Therefore, based on the application potential of proteomics in various aspects, the research of proteomics has great prospects and significance.

Fig.2 BIAchip™ in the process of neurobiology mechanism research

Please select the proteomics-related services you require below.

• Protein Identification and Quantification Analysis Service Based on SPR
• Peptidomic Analysis Service Based on SPR
• Protein Post-translational Modification Analysis Service Based on SPR
• Proteolysis Targeting Chimeras Research Based on SPR

If you are not interested in the direction you want to go, you can make an online inquiry for your project direction and you will receive a dedicated service.

Choosing SPR technology platform of Creative Proteomics, you will greatly save time and money costs owing to high-throughput intermolecular interaction detection. All services are available on a 24/7/365 basis. If you have any questions or suggestions about SPR, please feel free to contact us right now.

For research use only. Not intended for any clinical use.