SPR Technology for Kinetic Analysis

The role of kinetic analysis in basic research

Reaction kinetics is the study of the influence of various physical and chemical factors (such as temperature, pressure, concentration, medium in the reaction system, catalyst, etc.) on the reaction rate and the corresponding reaction mechanism. The chemical reaction rate constant k(T) and the chemical equilibrium constant Kc are important parameters describing the kinetics of chemical reactions.

• k(T)

The chemical reaction rate constant K(T) is one of the quantitative expressions of the chemical reaction rate. For the chemical reaction in which reactant A and reactant B react to form product C, the reaction rate can be expressed as this formula.

k(T) is the reaction rate constant, which changes with temperature.

m and n are called the reaction order depending on the reaction mechanism.

• Kc

The chemical equilibrium constant means that at a certain temperature, the reversible reaction starts from the positive reaction or the reverse reaction, regardless of the initial concentration of the reactants, and finally reaches equilibrium. At this time, the ratio Kc of the product of the stoichiometric power of the concentration of each product and the product of the stoichiometric power of the concentration of each reactant is called the chemical equilibrium constant. For the reversible chemical reaction mA+nB⇋pC+qD reaches chemical equilibrium at a certain temperature, the equilibrium constant expression is as this formula.

Surface plasmon resonance (SPR) system in the process of kinetic analysis

Kinetic analysis based on the determination of chemical reaction rate and chemical reaction equilibrium constant occupies an important position in the characterization of molecular interaction processes, and is mainly used to describe the rate of binding and dissociation between the analyte and the ligand. At present, kinetic analysis has been widely used in the process of drug development. We can judge the time and effect of the action of the bound drug molecule by detecting the binding or dissociation rate.

• Choosing our SPR services, first of all, you can obtain all the kinetic data of the sample you want to measure in a single run within a few minutes because of the high-throughput performance of SPR.
• Secondly, we can provide you with chip customization services according to your needs. Due to the design flexibility of SPR, you don't need to worry about your needs being rejected.
• Finally, the sensitivity and high selectivity of SPR services provide a guarantee for the accuracy of the final kinetic analysis result.

Fig.1 BIAchip™ in the process of kinetic analysis

Choosing SPR services of Creative Proteomics, you will greatly save time and money costs owing to high-throughput intermolecular interaction detection. All services are available on a 24/7/365 basis. If you have any questions or suggestions about our services, please feel free to contact us right now.

For research use only. Not intended for any clinical use.