SPR Technology for Concentration Analysis

The role of concentration analysis in basic research

Concentration analysis is the most basic property of a sample. In analysis and research, it is often necessary to determine the concentration of different components of the sample. Concentration determination is mainly used in the process of quality detection and identification. The determination of the biologically relevant activity concentration of biomolecules has been widely involved in people's daily life. For example, in drug development, we need to determine the effective content of drugs, in the field of food safety, we need to identify food safety, and in the field of environmental protection, we need to monitor the content of pollutants. All in all, in addition to basic research, concentration analysis is essential in other aspects.

Surface plasmon resonance (SPR) system in the process of concentration analysis

• The method of concentration analysis

(1) Direct Testing

Direct detection is mainly used for concentration analysis of high molecular weight samples. As shown in the schematic diagram below, This method is mainly used to detect the absolute response results of the sample to be tested and the ligand on the chip inside the instrument. Finally, a standard curve of sample concentration versus signal intensity can be obtained.

(2) Indirect Testing

Indirect detection is suitable for the detection of low molecular weight samples. Indirect detection is mainly by mixing a known amount of ligand molecules with the sample to be tested, and then detecting the concentration of the remaining ligand molecules through the SPR technology. Then a standard curve of unbound ligand concentration and signal intensity will be established to achieve indirect detection of sample concentration.

Fig.2 The method of concentration analysis based on the SPR technology

• SPR system in the process of concentration analysis

Active concentration analysis is the most basic content identification of sample properties. First of all, SPR technology platform can achieve high-throughput concentration determination of a large number of samples, which greatly reduces the waste of time. Secondly, we can provide customized chips corresponding to the above two detection methods to meet your needs for detection of samples with different molecular weights. Thirdly, the high sensitivity of the technology platform can reduce the detection line of the sample and increase the accuracy of the detection result. SPR technology platform is your best choice to achieve accurate concentration analysis.

Fig.3 BIAchip™ in the process of concentration analysis

Choosing SPR services of Creative Proteomics, you will greatly save time and money costs owing to high-throughput intermolecular interaction detection. All services are available on a 24/7/365 basis. If you have any questions or suggestions about our services, please feel free to contact us right now.

For research use only. Not intended for any clinical use.