Signaling Pathways in Metabolic Disease Analysis Service

Cellular metabolism is the sum of all the biochemical reactions that take place in the cell. These reactive processes enable organisms to grow, reproduce, maintain their structure and respond to the external environment. The molecular mechanisms behind various physiological phenomena, how organisms respond to environmental changes and how perturbations in physiological homeostasis can lead to disease are currently the focus of attention in the field of cellular metabolism research. Intensive studies of cellular metabolic signalling pathways play a key role in elucidating the mechanisms underlying many diseases. Metabolic diseases are caused by the accumulation or deficiency of certain metabolic substances, such as sugars, fats, proteins, purines as well as calcium and copper, which can disrupt biochemical processes in the body. Symptoms vary in severity and diagnosis relies on clinical manifestations and biochemical tests such as blood and urine.

Fig.1 Cellular responses to an array of cytokines and hormones utilize the JAK/STAT signal transduction pathway (Gurzov, E. N.; et al, 2016)

• Diseases of organelle metabolism

There are three main categories of organelle metabolic diseases including mitochondrial diseases, lysosomal diseases and peroxisomal diseases. Mitochondrial diseases are caused by mutations in mitochondrial DNA and are characterised by abnormal energy metabolism, mainly manifested by muscle weakness. Lysosomal storage disorders are a group of inherited metabolic disorders. Defective activity of lysosomal enzymes that fail to degrade the corresponding substrate leads to the deposition of that substrate in the lysosome, ultimately causing dysfunction of cells, tissues and organs.

• Substance metabolism disorders

Types of Substance Metabolism Disorders	Main Symptoms
Amino acid metabolic disorder	Phenylketonuria (abnormal phenylalanine metabolism disorder), glycine metabolic disorder, tyrosine metabolic disorder, etc.
Organic acid metabolic disorder	Methylmalonic acidemia, methylmalonic acidemia with homocysteinemia, propionic acidemia, etc.
Abnormal glucose metabolism disorder	Glycogen accumulation disorder ,galactosemia, etc.
Abnormal lipid metabolism disorder	Hypercholesterolaemia, hyperlipidaemia, etc.
Abnormal diseases of purine metabolism	Gout, autosomal disorder, etc.
Abnormal disorders of pigment metabolism	Porphyrias, etc
Abnormal diseases of blood ammonia metabolism	Hyperammonemia, histrionic protein deficiency, etc

Surface plasmon resonance (SPR) for Signaling pathways in metabolic disease analysis service

Metabolism is an essential condition for the normal life activity of an organism. When there is a problem with metabolic function, waste products are not properly removed from the cells and this in turn produces disease. Metabolic diseases, as chronic diseases, may have a slightly lower mortality rate than other diseases, but they are particularly disruptive to human life. Superficially, metabolic diseases are caused by problems with the activity of certain enzymes. However, in terms of underlying mechanisms, they are caused by problems with the signalling pathways that regulate enzyme activity. Therefore, the mechanisms of the signalling pathways involved in the study of metabolic diseases occupy an important place. As shown in the diagram below, metabolic disorders include abnormalities in amino acid metabolism, abnormalities in glucose metabolism and abnormalities in lipid metabolism. If you have research needs related to metabolic diseases, please consider our SPR technology platform. The SPR technology platform can be applied to the study of metabolic disease signaling mechanisms to drive the drug discovery process by targeting signal transduction pathways through high-throughput intermolecular interaction analysis.

Fig.2 BIAchip™ in the process of metabolic disease analysis service

In unravelling the mechanisms of metabolic disease at the molecular level, our technology platform has the following advantages.

• High throughput analysis
• High sensitivity analysis
• Highly flexible analysis

The ability to analyse hundreds or thousands of samples in parallel in a single run greatly increases efficiency and helps you to get the data and results you need in the shortest possible time. What's more, this high efficiency is achieved with a high degree of accuracy. You don't need to worry about the accuracy of the molecular interactions data obtained from our SPR services. In addition to this, the high flexibility of SPR technology allows us to offer you exclusive customisation, in accordance with your project requirements.

Choosing SPR services of Creative Proteomics, you will greatly save time and money costs owing to high-throughput intermolecular interaction detection. All services are available on a 24/7/365 basis. If you have any questions or suggestions about SPR, please feel free to contact us right now.

For research use only. Not intended for any clinical use.