Signaling Pathways in Cardiovascular Disease Analysis Service

which are ischaemic or haemorrhagic disorders of the heart, brain and body tissues caused by hyperlipidaemia, blood viscosity, atherosclerosis and hypertension. Cardiovascular disease is a common disease that poses a serious threat to human health, especially in middle-aged and elderly people over the age of 50, and is characterised by high morbidity, disability and mortality. Cardiovascular disease is a manifestation of systemic vascular disease or systemic vascular disease in the heart and brain. There are four main causes of cardiovascular disease.

(1) Vascular factors, such as atherosclerosis, hypertension small arteriosclerosis and arteritis.

(2) Haemodynamic factors, such as hypertension.

(3) Blood rheological abnormalities, such as hyperlipidaemia and diabetes mellitus.

(4) Blood component factors, such as leukaemia, anaemia and thrombocytosis.

Fig.1 Schematic illustration of atherosclerosis development. (Chae, C.-W.; et al, 2020)

• eNOS/NO signaling pathway

Nitric oxide (NO) is a signaling molecule that plays an important role in physiological activities, including blood pressure regulation, maintenance of vascular tone and regulation of the immune system. Endothelial nitric oxide synthase (eNOS), the rate-limiting enzyme that induces nitric oxide synthesis, plays an important role in the regulation of the vascular wall and is therefore closely associated with normal cardiovascular physiological activity.

• Hippo-YAP signaling pathway

The Hippo-YAP pathway plays an important role in the regulation of cell proliferation and apoptosis and its core members include proteins such as YAP. The Hippo-YAP signaling pathway plays an important role in tumor infiltration and metastasis, stem cell differentiation and cardiovascular disease by influencing cell proliferation and other processes.

• mTOR signaling pathway

The mTOR kinase plays an important role in the regulation of intracellular homeostasis and growth in developing and postnatal cardiomyocytes. mTOR knockout mice show death in early embryonic development. Embryos with cardiac-specific mTOR knockouts exhibit reduced cardiomyocyte proliferation, increased apoptosis, cardiac dilation and cardiac dysfunction, ultimately leading to heart failure.

Surface plasmon resonance (SPR) for signaling pathways in cardiovascular disease analysis

Based on the introduction of cardiovascular disease mentioned above, it is well known that it is currently the most prevalent disease and usually occurs in the elderly. This disease often turns from chronic to acute and ultimately outright fatal as we age. Many signaling pathways are involved in the regulation of the normal vital activities of the heart and brain, providing for the normal physiological activities of the living body. Therefore, cardiovascular disease often arises from a problem in the regulation of one or more signaling pathways. Research into the mechanisms of these various signaling pathways involved in cardiovascular disease provides new ideas for the development of cardiovascular drugs and is a potential target for cardiovascular drug development. If you have a research need in this area, you might consider taking a look at our technology platform. As you can see from the diagram below, SPR technology can be used not only for the discovery of new signaling pathways during cardiovascular disease research, but also for the development of relevant drugs.

Fig.2 BIAchip™ in the process of signaling pathways in cardiovascular disease analysis

• Research on signaling pathway mechanisms in cardiovascular disease

The analysis of interactions between biomolecules is fundamental to the study of signaling pathway mechanisms. SPR technology is suitable for the analysis of almost all biomolecular interactions due to its flexibility and scalability. In addition to standard templates for you to choose from, we also offer you a customised service. After placing an order, you will receive feedback from our experts on your project requirements within 2-3 days.

• Cardiovascular disease signaling pathways as potential targets

Mechanistic studies on signaling pathways in cardiovascular disease can provide new ideas and potential targets for relevant drug development. The high-throughput SPR technology platforms can provide you with high-throughput target selection and screening to advance the drug discovery process.

• Drug screening for cardiovascular disease

Once the target of the signaling pathway has been identified, our SPR technology platform can also provide target-based high-throughput screening services. Based on data from rapid detection of affinity and kinetic results, preliminary screening of drugs can be achieved.

Choosing SPR technology platform of Creative Proteomics, you will greatly save time and money costs owing to high-throughput intermolecular interaction detection. All services are available on a 24/7/365 basis. If you have any questions or suggestions about our SPR services, please feel free to contact us right now.

For research use only. Not intended for any clinical use.