Protein-Small Molecule Interaction Chip

Category
Capture sensor chip

Description
His-tagged molecules are immobilized via Ni2+/NTA chelationimple and surface regeneration is done by injecting EDTA or imidazole. 200 nm coating thickness.

Feature
Binding is reversible and his-tagged ligand can be removed using a chelating agent (e.g. EDTA)
Results in an oriented capture
High capacity for histidine-tagged molecules such as recombinant proteins

Category
Capture sensor chip

Description
The interaction between streptavidin and biotin results in a high-affinity biomolecular interaction with negligible dissociation of the ligand from the sensor chip surface, making it an ideal choice for high-capacity, reproducible SPR experimentation. Minimal biotinylation of the ligand and subsequent capture on a Streptavidin/Dextran chip results in a more oriented arrangement of ligand molecules on the surface as compared to the more random arrangement from chemical immobilization such as amine coupling and also offers a higher capacity than a planar chip.

Feature
Medium to high capacity for biotinylated ligand captures
Binding resistant to a wide range of conditions including heat, pH, and proteolysis
This surface is used for the high-affinity capture of biotinylated molecules such as proteins, peptides, and nucleic acids

Category
Covalently coupled biosensor chip

Description
Carboxymethyl dextran surfaces are very stable and resistant to the non-specific binding of biomolecules. The dextran layer is in the form of a highly flexible non-cross-linked brush-like structure extending 100 to 200 nanometers from the surface. The flexible nature of the dextran contributes to the accessibility of binding sites on an immobilized ligand. 3D carboxymethyl dextran sensors coated with dextran hydrogel layer of low, medium or high density. Medium density, 500 and 700 nm coating thickness.

Feature
High binding capacity
Produces highly stable covalent bond
3D carboxymethyl dextran sensors with medium density are recommended for most biological assays like protein-small molecule or protein-protein interactions

Category
Covalently coupled biosensor chip

Description
Polycarboxylate hydrogel sensor chip. Medium density, 150, 1000 and 1500 nm coating thickness.

Feature
Use for large/medium sized molecules and low molecular weight molecules
Provides another choice for high capacity coupling when studying protein-small molecule interactions or when dextran is not ideal