Protein-Protein Interaction Chip

Category
Capture sensor chip

Description
Native immobilization via Fc-domain affinity. This product combines protein A and protein G to bind a wide range of antibodies from many mammalian species and almost all subclasses of human immunoglobulins.

Feature
High capacity for quantitation of multi-species IgG

Category
Capture sensor chip

Description
Protein A contains four high-affinity binding sites capable of binding lgG (predominantly human) through the Fc-region.

Feature
Optimal binding occurs at pH 8.2, although binding is also effective at neutral or physiological conditions (pH 7.0 to 7.6)

Category
Capture sensor chip

Description
Targeted capture or binding of a broad range of mammalian and all human antibody subclasses.

Feature
For antibody interaction analysis

Category
Capture sensor chip

Description
His-tagged molecules are immobilized via Ni2+/NTA chelationimple and surface regeneration is done by injecting EDTA or imidazole.

Feature
Binding is reversible and his-tagged ligand can be removed using a chelating agent (e.g. EDTA)
High capacity for histidine-tagged molecules such as recombinant proteins and works well even for protein-small molecule interactions

Category
Capture sensor chip

Description
The binding of NeutrAvidin to biotin is one of the strongest non-covalent interactions. Minimal biotinylation of the ligand and subsequent capture on a NeutrAvidin chip results in a more oriented arrangement of ligand molecules on the surface as compared to the more random arrangement from chemical immobilization such as amine coupling.

Feature
Minimal biotinylation of the ligand and subsequent capture results in a more oriented arrangement of ligand molecules on the surface
This surface is used for the high-affinity capture of biotinylated molecules such as proteins, peptides, and nucleic acids

Category
Capture sensor chip

Description
The interaction between streptavidin and biotin results in a high-affinity biomolecular interaction with negligible dissociation of the ligand from the sensor chip surface, making it an ideal choice for high-capacity, reproducible SPR experimentation. Minimal biotinylation of the ligand and subsequent capture on a Streptavidin/Dextran chip results in a more oriented arrangement of ligand molecules on the surface as compared to the more random arrangement from chemical immobilization such as amine coupling and also offers a higher capacity than a planar chip.

Feature
Medium to high capacity for biotinylated ligand captures
Binding resistant to a wide range of conditions including heat, pH, and proteolysis
This surface is used for the high-affinity capture of biotinylated molecules such as proteins, peptides, and nucleic acids

Category
Capture sensor chip

Description
Lipophilic alkyl chains are linked to a planar carboxymethyl dextran layer. Such 2D structures can be simply prepared by vesicle spreading and mimic the cell membrane.

Feature
Supported lipid bilayers and transmembane proteins
Suitable for analysis of membrane protein-protein interactions or membrane protein-inhibitor interactions

Category
Capture sensor chip

Description
Carboxymethylated dextran hydrogel with lipophilic groups covalently attached. For interaction analysis involving transmembrane receptors in membrane-like environments. 

Feature
Enable stable, high-capacity capture of whole vesicles and liposomes while maintaining the lipid bilayer
Suitable for analysis of membrane protein-protein interactions or membrane protein-inhibitor interactions

Category
Covalently coupled biosensor chip

Description
Carboxymethyl dextran surfaces are very stable and resistant to the non-specific binding of biomolecules. The dextran layer is in the form of a highly flexible non-cross-linked brush-like structure extending 100 to 200 nanometers from the surface. The flexible nature of the dextran contributes to the accessibility of binding sites on an immobilized ligand. Planar carboxymethyl dextran coating (less than 5 nm) allows the immobilization of ligands in a single monolayer.

Feature
2D planar carboxymethyldextran surface, <5nm coating thickness
Covalent coupling
Recommended for the binding of high molecular weight analytes such as antibodies or nanoparticles
The CMDP coating is also suitable for particulate analytes, such as viruses and whole cells

Category
Covalently coupled biosensor chip

Description
Carboxymethyl dextran surfaces are very stable and resistant to the non-specific binding of biomolecules. The dextran layer is in the form of a highly flexible non-cross-linked brush-like structure extending 100 to 200 nanometers from the surface. The flexible nature of the dextran contributes to the accessibility of binding sites on an immobilized ligand. 3D carboxymethyl dextran sensors coated with dextran hydrogel layer of low, medium or high density. low - medium density, 50, 200, 500 nm coating thickness.

Feature
High binding capacity
Produces highly stable covalent bond
Medium density (3DM) is recommended for most biological assays like protein-small molecule or protein-protein interactions.

Category
Covalently coupled biosensor chip

Description
Polycarboxylate hydrogel sensor chip 30, 200, 1000 nm coating thickness, low-medium density.

Feature
Provides another choice for high capacity coupling when studying protein-small molecule interactions or when dextran is not ideal