Protein-DNA Interaction Chip

Category
Capture sensor chip

Description
The binding of NeutrAvidin to biotin is one of the strongest non-covalent interactions. Minimal biotinylation of the ligand and subsequent capture on a NeutrAvidin chip results in a more oriented arrangement of ligand molecules on the surface as compared to the more random arrangement from chemical immobilization such as amine coupling. Neutravidin derivatized carboxymethyl dextran. May reduce the non-specific binding for positively charged analytes. Low-medium density, 50, 200, 500 nm coating thickness.

Feature
This surface is used for the high-affinity capture of biotinylated molecules such as proteins, peptides, and nucleic acids
Minimal biotinylation of the ligand and subsequent capture results in a more oriented arrangement of ligand molecules on the surface
An alternative for SA sensor when there is non-specific binding

Category
Capture sensor chip

Description
The binding of NeutrAvidin to biotin is one of the strongest non-covalent interactions. Neutravidin derivatized linear polycarboxylate. May reduce non-specific binding for positively charged analytes. 30 and 200 nm coating thickness, medium density.

Feature
Low-medium density

Category
Capture sensor chip

Description
The interaction between streptavidin and biotin results in a high-affinity biomolecular interaction with negligible dissociation of the ligand from the sensor chip surface, making it an ideal choice for high-capacity, reproducible SPR experimentation. Minimal biotinylation of the ligand and subsequent capture on a Streptavidin/Dextran chip results in a more oriented arrangement of ligand molecules on the surface as compared to the more random arrangement from chemical immobilization such as amine coupling and also offers a higher capacity than a planar chip. Low-medium density, 50, 200, 500 nm coating thickness.

Feature
Medium to high capacity for biotinylated ligand captures
Binding resistant to a wide range of conditions including heat, pH and proteolysis
This surface is used for the high-affinity capture of biotinylated molecules such as proteins, peptides, and nucleic acids

Category
Capture sensor chip

Description
The binding of Streptavidin to biotin is one of the strongest non-covalent interactions. This high-affinity streptavidin (SA) sensor chip immobilizes biotinylated molecules for SPR interaction analysis. Streptavidin, immobilized in a polycarboxylate hydrogel, 200 nm coating thickness, medium density.

Feature
Lower capacity for biotinylated ligand kinetic lawns

Category
Covalently coupled biosensor chip

Description
Polycarboxylate hydrogel sensor chip 30, 200, 1000 nm coating thickness, low-medium density.

Feature
Provides another choice for high capacity coupling when studying protein-small molecule interactions or when dextran is not ideal