Nucleic Acid Drug Research Based on SPR Technology

The overview of nucleic acid drugs research

Nucleic acid drugs are nucleic acids themselves or closely related compounds that can be used to treat diseases, including natural nucleotides and chemically modified nucleotides. Nucleic acid drugs include antisense nucleic acid (ASO), small interfering RNA (siRNA), microRNA (miRNA), small activating RNA (saRNA), aptamer, ribozyme, antibody nucleic acid conjugated drug (ARC), which is a form of gene therapy and a new generation of pharmaceutical technology. Depending on the target and mechanism of action, there are nucleic acid drugs with different types and characteristics. The details are shown in the table below. Although there are various types of nucleic acid drugs, they all share a common mechanism of action, which specifically recognizes endogenous nucleic acid sequences through the Watson-Crick base pairing mechanism. In addition to gene therapy, nucleic acids used for therapy can also inhibit the expression of abnormal proteins associated with diseases by inhibiting the expression of DNA or RNA, without affecting the expression of other proteins. Compared with antibody drugs, nucleic acid drugs show more efficacy and safety than antibody drugs, and because of their relatively small molecular weight, it is conducive to mass production by pharmaceutical companies. These characteristics make nucleic acid drugs promising for previously difficult-to-treat cancers and genetic diseases, as well as diseases caused by viral infections such as influenza.

Type	Target	Mechanism	Summary
siRNA	mRNA	mRNA cleavage	According to the principle of RNAi, double-stranded RNA or single-stranded hairpin RNA complementary to the target mRNA sequence
miRNA	microRNA	microRNA replacement	miRNAs of double-stranded RNA, single-stranded hairpin RNA, or their analogs are used to enhance the function of miRNAs that are exacerbated by dysregulation
Antisense Nucleic Acid	mRNA、miRNA	mRNA and miRNA degradation, inhibition of splicing	Single-stranded RNA/DNA binds to target mRNAs and miRNAs resulting in degradation or repression, or exon skipping during splicing
Aptamer	Extracellular Protein	Functional inhibition	Single-stranded RNA/DNA binds to target protein in a manner similar to antibody/DNA
RNA Decoys	Transcription Factor	RNA cleavage	Double-stranded DNA with the same sequence as the transcription factor binding site, binds to the transcription factor of the affected gene and represses the target gene
Ribozyme	RNA	RNA cleavage	Single-stranded RNA has the enzymatic function of binding and cleaving target RNA
CPG Oligo	Protein Receptor	Induction of immunity	Oligonucleotides with CpG sequences

Surface plasmon resonance (SPR) in the process of nucleic acid drugs research

Nucleic acid drugs mainly play a role in treating diseases at the gene level by directly acting on pathogenic target genes or target mRNAs, and fundamentally solve problems. Compared with traditional drugs that work at the protein level, nucleic acid drugs have obvious advantages of high efficiency and long-term effect. Therefore, the research on nucleic acid drugs is very meaningful. In the study of nucleic acid drugs, the part involving molecular interactions is mainly nucleic acid-related interaction analysis, such as DNA-DNA, DNA-RNA, RNA-RNA, RNA-protein, etc. If you have research needs in this area, please pay attention to our SPR technology platform. The general process of SPR technology participating in the development of nucleic acid drugs is shown in the figure below, which can greatly simplify your operation process in the process of molecular interaction experiments.

Fig.1 BIAchip^TM in the process of nucleic acid drugs research

First of all, the SPR technology platform can provide you with high-throughput nucleic acid-related interaction analysis services, which greatly promotes the entire research process of nucleic acid drugs. Then, based on the high sensitivity of the platform, the low detection limit of SPR technology can ensure the accuracy of the whole process of nucleic acid-related interaction analysis, which you need not worry about. In addition, we provide you with a variety of nucleic acid-related interaction analysis services to choose from. If you do not find the type of analysis service suitable for your experimental project, please feel free to contact us for exclusive customized services.

Choosing the SPR technology platform of Creative Proteomics, you will greatly save time and money costs owing to high-throughput intermolecular interaction detection. All services are available on a 24/7/365 basis. If you have any questions or suggestions about our SPR platform, please feel free to contact us right now.

For research use only. Not intended for any clinical use.