DNA-DNA, Small Molecule, Peptide Interaction Chip

Category
Capture sensor chip

Description
The interaction between streptavidin and biotin results in a high-affinity biomolecular interaction with negligible dissociation of the ligand from the sensor chip surface, making it an ideal choice for high-capacity, reproducible SPR experimentation. Minimal biotinylation of the ligand and subsequent capture on a Streptavidin/Dextran chip results in a more oriented arrangement of ligand molecules on the surface as compared to the more random arrangement from chemical immobilization such as amine coupling and also offers a higher capacity than a planar chip. 500 nm coating thickness, medium density.

Feature
Medium to high capacity for biotinylated ligand captures
Binding resistant to a wide range of conditions including heat, pH, and proteolysis
This surface is used for the high-affinity capture of biotinylated molecules such as proteins, peptides, and nucleic acids

Category
Capture sensor chip

Description
The binding of Streptavidin to biotin is one of the strongest non-covalent interactions. This high-affinity streptavidin (SA) sensor chip immobilizes biotinylated molecules for SPR interaction analysis. Streptavidin, immobilized in a Polycarboxylate hydrogel, 1000 nm coating thickness, medium density.

Feature
Lower capacity for biotinylated ligand kinetic lawns