Cytokine Detection Service Based on SPR Technology

Cytokines are small molecular peptides or glycoproteins synthesised and secreted by immune cells, such as monocytes, macrophages, T cells, B cells and NK cells. Cytokines mediate cellular interactions and have a variety of biological functions, such as regulating cell growth, cell differentiation, maintenance of cellular function, modulation of the immune response, involvement in inflammatory responses, wound healing and tumour regression. There are several categories of cytokines, including interleukins, interferons, tumour necrosis factor superfamily, colony-stimulating factors, chemokines, growth factors and others. As we can see in the diagram below, cytokines act in the body by paracrine, autocrine or endocrine means and have a variety of physiological properties such as pleiotropy, overlap, antagonism and synergism. Cytokines form a very complex cytokine regulatory network and are involved in a variety of important physiological functions in the body. Cytokines not only act on the immune and haematopoietic systems, but also on the neurological and endocrine systems, and play an important role in regulating cellular interactions, cell proliferation and differentiation, and effector functions. Cytokines perform a wide range of biological functions primarily by binding to receptors on the surface of target cell membranes and transmitting signals to the cell interior. Therefore, the study of the function and interaction mechanisms of cytokines and cytokine receptors is essential for an in-depth study of the biological functions of cytokines.

Fig.1 Mode of action of cytokines (A: Autocrine;B: Paracrine; C: Endocrine)

Surface plasmon resonance (SPR) in the process of cytokine detection

Cytokines occupy an important position in the immune system of living organisms as small molecular proteins that are important information transducers. The study of the mechanism of action of cytokines has important implications for the study of the function of the immune system and new approaches to immunotherapy. As we can see in the left part of the diagram below, the mechanism of action of cytokines is broadly divided into three stages. Firstly, external stimuli trigger an immune response in the immune system and immune cells release cytokines. Secondly, the cytokines interact with cytokines on target cells to stimulate a downstream response. Thirdly, the target cell receives the signal from the cytokine and produces the corresponding effect. It is clear that all three stages involve protein-related interactions, so analysis of the interaction mechanism is a key step in the study of the next cytokine mechanism of action. Creative Proteomics can offer a high-throughput technology platform for the analysis of protein-related interactions called SPR. If your experimental project requires the study of the structure and function of cytokines, you may choose to try out the molecular interaction analysis services provided by our SPR technology platform.

Fig.2 BIAchip™ in the process of cytokine detection

The SPR technology platform has many outstanding advantages over traditional interaction analysis technology platforms, which is the reason you have to choose us.

• Immunology specialists will be available to meet with you on a one-to-one basis as soon as we receive your order in order to fully understand your needs. Due to the flexibility and scalability of the SPR technology platform, it is possible to meet essentially any of your customised needs. This is another important advantage of our technology platform in addition to the high throughput analysis. What's more, the customisation cycle of the chips will not take you too long and you will receive feedback within 1-2 days because of the expertise of our technical and scientific team.
• The high sensitivity of SPR technology facilitates the detection of samples with small molecular weights. Conventional molecular interaction analysis methods generally give weak or almost no signal for the detection of small molecular weight samples. Cytokines are small proteins and our technology platform is the right choice for easy detection and accurate results. Our SPR technology platform can provide high throughput and accuracy cytokine-receptor interaction analysis services to advance immune mechanism research and immunotherapy development.

Choosing SPR technology platform of Creative Proteomics, you will greatly save time and money costs owing to high-throughput intermolecular interaction analysis servive about cytokines. All services are available on a 24/7/365 basis. If you have any questions or suggestions about SPR, please feel free to contact us right now.

For research use only. Not intended for any clinical use.