Antigen-antibody Affinity Analysis Service Based on SPR Technology

Antigen-antibody affinity is the binding force between an antibody and an antigenic epitope or antigenic determinant cluster and is essentially a non-covalent force that incorporates attractive forces between amino acids, hydrogen bonding, hydrophobic forces, etc. Antigen-antibody affinity analysis plays an important role in the study of the mechanisms of infectious diseases and related specific drugs, and is a criterion and basis for the selection of antibody drugs. The affinity of an antigen-antibody can be expressed by the affinity constant KD, calculated as KD = [Ab-H]/([Ab][H]) where [] represents the molar concentration, Ab represents the antibody, H represents the semi-antigen and Ab-H represents the binding of the antibody to the semi-antigen. The higher the affinity constant KD, the greater the ability of the antibody to bind the semi-antigen.

Fig.1 Modelling of antibody-antigen complexes via the immunoglobulin pool. ( Raybould M.I.; et al, 2019)

The strength of antibody affinity depends on the degree of fit between the antibody counterpart and the epitope of the antigen used, including the size of the contact area, the degree of affinity and the distribution of the dotted groups and hydrophobic genes. Antibody affinity reflects the ability of an antibody molecule to react with a determinant cluster of antigen molecules or semi-antigens and is an important indicator of antibody quality, reflecting the strength of binding between the antibody and the corresponding antigen. The higher the affinity, the higher the degree of binding between the antibody and the corresponding antigen, and is an important guide to the application of antibodies.

Surface plasmon resonance (SPR) in the process of antigen-antibody affinity analysis

The affinity effect of an antigen-antibody is the basis for the screening of antibody drugs and is a key parameter of antibody drugs, which usually affects the function and efficacy of the antibody. In practical development, there are many aspects of the conventional screening of antibodies that require more detailed improvement, including affinity, immunogenicity, half-life, etc. Researchers have done a lot of research and practice in the field of antibodies over the years, with antibody affinity maturation being one of the key areas of research. Theoretically, increasing the affinity of antibodies can help improve the specificity and potency of antibodies, help reduce the dose of drugs and reduce toxic side effects. Furthermore, the development of antibody affinity maturation techniques will not only contribute to the development and quality improvement of antibody drugs, but also to a better understanding of the mechanisms of antibody-target interactions and better understanding of target function. Based on the importance of antigen-antibody affinity studies in antibody drug development, Creative Proteomics is committed to providing you with high-throughput antigen-antibody affinity analysis services through SPR technology.

Fig.2 BIAchip™ in the process of antigen-antibody affinity analysis

As shown above, our technology platform can provide you with high-throughput antigen-antibody affinity analysis services that ensure accuracy while greatly improving efficiency compared to traditional molecular interactions platforms.

• High efficiency

With the ability to perform hundreds of samples in a single run, SPR technology reduces the tedious work involved in screening antibodies and greatly improves your productivity. In addition to this, SPR technology allows you to extend the scope of your screening work to the widest possible range.

• Low cost

Firstly, high throughput screening significantly reduces costs. Secondly, our chips are reusable if handled and stored according to our instructions for use. Don't worry, our experts will advise you in detail on how to use and store the chips to prolong their life.

With so many significant advantages for antigen-antibody affinity analysis, why not try our SPR technology platform? Once you have placed your order, Creative Proteomics will arrange for you to receive a tailor-made service from our experts in target screening field. All services are available on a 24/7/365 basis. If you have any questions or suggestions about SPR, please feel free to contact us right now.

For research use only. Not intended for any clinical use.